Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

[Application of the duplex-specific nuclease for fast analysis of single nucleotide polymorphisms and detection of target DNA in complex PCR products].

Bioorganicheskaia khimiia - 1 Jan 2000

Shagina I A, Bogdanova E A, Al'tshuler I M, Luk'ianov S A, Shagin D A

Abstract excerpt

We have developed a simple method for fast analysis of single nucleotide polymorphisms and identification of target clones from cloned complex PCR products. The method utilizes Kamchatka crab duplex-specific nuclease and universal fluorescent probe and is alternative to laborious screening procedures using radioactive probes, restriction analysis followed by gel electrophoresis or expensive sequencing. The method...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Brachyura
Share to Cell Cycle Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cloning, Molecular

Share to Deoxyribonucleases

Share to DNA Mutational Analysis

Share to Nuclear Proteins

Share to Receptors, Antigen, T-Cell

Source

Identifiers

PMID: 22096995
DOI: 10.1134/s1068162011040121

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

[Application of the duplex-specific nuclease for fast analysis of single nucleotide polymorphisms and detection of target DNA in complex PCR products].PMID 22096995Read ↗

A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas.Semantic proximity Read ↗

DSN depletion is a simple method to remove selected transcripts from cDNA populations.Semantic proximity Read ↗

SNP genotyping by the 5'-nuclease reaction: advances in high-throughput genotyping with nonmodel organisms.Semantic proximity Read ↗

[PCR-SSCP--rapid and easy detection of DNA-sequence changes].Semantic proximity Read ↗

[Application of the duplex-specific nuclease preference method to the analysis of point mutations in human genes].Semantic proximity Read ↗

[Single-strand conformation polymorphism (SSCP) analysis: a convenient, rapid method for detection of single-base changes in DNA].Semantic proximity Read ↗

Advances in quantitative PCR technology: 5' nuclease assays.Semantic proximity Read ↗

Use of DNA toolbox for the characterization of mutation scanning methods. II: evaluation of single-strand conformation polymorphism analysis.Semantic proximity Read ↗

Scanning of nucleic acids by in vitro amplification: new developments and applications.Semantic proximity Read ↗

Microsatellite-containing genes from the channel catfish brain: evidence of trinucleotide repeat expansion in the coding region of nucleotide excision repair gene RAD23B.Semantic proximity Read ↗

[Nucleic acid syntheses and molecular recognition].Semantic proximity Read ↗

Mutation detection using Surveyor nuclease.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.