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Article

Advances in quantitative PCR technology: 5' nuclease assays.

Current opinion in biotechnology - 1 Feb 1998

Lie Y S, Petropoulos C J

Abstract excerpt

The development of 5' nuclease assays represents a significant advance in nucleic acid quantitation. This approach utilizes the 5'-3' exonuclease activity of Thermus aquaticus (Taq) polymerase to cleave a dual-labelled probe annealed to a target sequence during amplification. The release of a flu...

Topics

Alleles
Exodeoxyribonucleases
Gene Dosage
Kinetics
Polymerase Chain Reaction
RNA

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 9503586
DOI: 10.1016/s0958-1669(98)80082-7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9503586-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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