Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Advances in quantitative PCR technology: 5' nuclease assays.

Current opinion in biotechnology - 1 Feb 1998

Lie Y S, Petropoulos C J

Abstract excerpt

The development of 5' nuclease assays represents a significant advance in nucleic acid quantitation. This approach utilizes the 5'-3' exonuclease activity of Thermus aquaticus (Taq) polymerase to cleave a dual-labelled probe annealed to a target sequence during amplification. The release of a fluorogenic tag from the 5' end of the probe is proportional to the target sequence concentration (copy number), and can...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Exodeoxyribonucleases
Share to Gene Dosage

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Kinetics

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 9503586
DOI: 10.1016/s0958-1669(98)80082-7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Advances in quantitative PCR technology: 5' nuclease assays.PMID 9503586Read ↗

One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase.Semantic proximity Read ↗

Real-time PCR in virologySemantic proximity Read ↗

Comparison of DNA polymerases for quantification of single nucleotide differences by primer extension assays.Semantic proximity Read ↗

A novel PCR method directly quantifies sequence features that block primer extensionSemantic proximity Read ↗

Nuclease activity: an exploitable biomarker in bacterial infectionsSemantic proximity Read ↗

PCR-SSP: a cautionary tale and an update of the Bein primer mixes.Semantic proximity Read ↗

Real-time PCR: overview and applications.Semantic proximity Read ↗

Alternative nucleic amplification strategies, part 2: LCR and HDA.Semantic proximity Read ↗

Directed termination of the polymerase chain reaction: kinetics and applications in mutation detection.Semantic proximity Read ↗

Ligation with nucleic acid sequence-based amplification.Semantic proximity Read ↗

New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Forster resonance energy transfer probes in 5'-nuclease assays.Semantic proximity Read ↗

A 5′-nuclease real-time reverse transcriptase–polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.