Article
A polybasic domain or palmitoylation is required in addition to the CAAX motif to localize p21ras to the plasma membrane.
Cell - 5 Oct 1990
Hancock J F, Paterson H, Marshall C J
Abstract excerpt
The C-terminal CAAX motif of ras proteins undergoes a triplet of posttranslational modifications that are required for membrane association. The CAAX motif lies immediately C-terminal to the hypervariable domain, a region of 20 amino acids that distinguishes the ras proteins from each other. The hypervariable domains of p21H-ras, p21N-ras, and p21K-ras(A) contain sites for palmitoylation, which we now show must...
Topics
- Amino Acid Sequence
- Animals
- Cell Line
- Cell Membrane
- Fluorescent Antibody Technique
- Methionine
- Mice
- Mutation
- Palmitic Acids
- Proto-Oncogene Proteins p21(ras)
- Transfection