Article
Microbody phosphoglycerate kinase of Trypanosoma brucei: expression and complementation in Escherichia coli.
Gene - 15 Jun 1990
Alexander K, Hill T, Schilling J, Parsons M
Abstract excerpt
In the primitive eukaryotic parasite, Trypanosoma brucei, most of the enzymes of glycolysis are located within microbody organelles called glycosomes. Proteins destined for the glycosome are synthesized on free ribosomes and post-translationally translocated into the organelle. The gene, gPGK, encoding the glycosomal isozyme of phosphoglycerate kinase (gPGK), was cloned adjacent to a T7 promoter and cotransformed...
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