Article
Complementation of an Escherichia coli glycolysis mutant by Giardia lamblia triosephosphate isomerase.
Experimental parasitology - 1 Feb 1994
Mowatt M R, Weinbach E C, Howard T C, Nash T E
Abstract excerpt
The Giardia lamblia triosephosphate isomerase (TIM) gene was cloned using a probe generated by a polymerase chain reaction that employed primers complementary to highly conserved regions of TIM. The nucleotide sequence predicts a protein that is 38 and 47% identical to TIM from prokaryotic and eu...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- Escherichia coli
- Gene Expression Regulation
- Genes, Protozoan
- Genetic Complementation Test
- Giardia lamblia
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Plasmids
- Polymerase Chain Reaction
- RNA, Messenger
- Transfection
- Triose-Phosphate Isomerase