Article
Molecular cloning and analysis of two tandemly linked genes for pyruvate kinase of Trypanosoma brucei.
European journal of biochemistry - 15 Aug 1991
Allert S, Ernest I, Poliszczak A, Opperdoes F R, Michels P A
Abstract excerpt
In Trypanosoma brucei (stock 427) genes encoding the glycolytic enzyme pyruvate kinase are present on two homologous chromosomes. We have cloned and characterized one of the alleles. Two large, tandemly arranged open reading frames were found, each coding for a pyruvate kinase polypeptide of 498...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA, Protozoan
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Humans
- Molecular Sequence Data
- Open Reading Frames
- Pyruvate Kinase
- Repetitive Sequences, Nucleic Acid
- Saccharomyces cerevisiae
- Sequence Alignment
- Sequence Homology, Nucleic Acid
- Transcription, Genetic