Cloning metabolic pathway genes by complementation in Escherichia coli. Isolation and expression of Plasmodium falciparum glucose phosphate isomerase.

Abstract excerpt

Genetic complementation of an Escherichia coli double mutant was used to isolate and express the gene coding for Plasmodium falciparum glucose phosphate isomerase. The gene contains a 1773-base pair open reading frame, has no introns, and maps to P. falciparum chromosome 14. 34% of the deduced amino acid sequence is identical to human glucose phosphate isomerase, with highest similarity in regions of the proposed...

Topics

• Animals
• Base Sequence
• Blotting, Southern
• Cloning, Molecular
• Codon
• Escherichia coli
• Gene Expression
• Genes
• Genetic Complementation Test
• Glucose-6-Phosphate Isomerase
• Molecular Sequence Data