Article
Cloning by function: an alternative approach for identifying yeast homologs of genes from other organisms.
Proceedings of the National Academy of Sciences of the United States of America - 1 Sept 1990
Kranz J E, Holm C
Abstract excerpt
Studies of cell physiology and structure have identified many intriguing proteins that could be analyzed for function by using the power of yeast genetics. Unfortunately, identifying the homologous yeast gene with the two most commonly used approaches--DNA hybridization and antibody cross-reaction--is often difficult. We describe a strategy to identify yeast homologs based on protein function itself. This...
Topics
- Base Sequence
- Cloning, Molecular
- Gene Conversion
- Genes, Fungal
- Genetic Complementation Test
- Genotype
- Mutation
- Plasmids
- Saccharomyces cerevisiae
- Sequence Homology, Nucleic Acid