Article
Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli.
The Journal of biological chemistry - 25 Aug 1990
Polesky A H, Steitz T A, Grindley N D, Joyce C M
Abstract excerpt
The Klenow fragment structure, together with many biochemical experiments, has suggested a region of the protein that may contain the polymerase active site. We have changed 7 amino acid residues within this region by site-directed mutagenesis, yielding 12 mutant proteins which have been purified...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- DNA Polymerase I
- Deoxyribonuclease I
- Escherichia coli
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Peptide Fragments
- Protein Conformation
- Sequence Homology, Nucleic Acid