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Article

Side chains involved in catalysis of the polymerase reaction of DNA polymerase I from Escherichia coli.

The Journal of biological chemistry - 25 Apr 1992

Polesky A H, Dahlberg M E, Benkovic S J, Grindley N D, Joyce C M

Abstract excerpt

To continue our mutagenesis study of the polymerase active site of the Klenow fragment of DNA polymerase I, we have characterized new mutants with substitutions at Asp705, Glu710, and Glu883, and have investigated further the mutations in residues Arg668, Gln849, and Asp882, which showed strong Kcat effects in our previous study. To determine which step of the reaction is rate-limiting for each mutant protein, we...

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PMID: 1569092

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