Side chains involved in catalysis of the polymerase reaction of DNA polymerase I from Escherichia coli.

Article

Side chains involved in catalysis of the polymerase reaction of DNA polymerase I from Escherichia coli.

The Journal of biological chemistry - 25 Apr 1992

Polesky A H, Dahlberg M E, Benkovic S J, Grindley N D, Joyce C M

Abstract excerpt

To continue our mutagenesis study of the polymerase active site of the Klenow fragment of DNA polymerase I, we have characterized new mutants with substitutions at Asp705, Glu710, and Glu883, and have investigated further the mutations in residues Arg668, Gln849, and Asp882, which showed strong K...

Topics

Amino Acids
Base Sequence
Catalysis
DNA Polymerase I
DNA, Bacterial
Deoxyribonucleotides
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Kinetics
Molecular Sequence Data
Mutation

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