Article
Broad spectrum of in vivo forward mutations, hypermutations, and mutational hotspots in a retroviral shuttle vector after a single replication cycle: substitutions, frameshifts, and hypermutations.
Proceedings of the National Academy of Sciences of the United States of America - 1 Aug 1990
Pathak V K, Temin H M
Abstract excerpt
We determined the in vivo forward mutation rate in a single replication cycle for spleen necrosis virus (SNV). A method was developed to clone integrated proviruses of retroviral shuttle vectors by exploiting the tight binding of the lac operator to the lac repressor protein. The vectors containe...
Topics
- Animals
- Base Sequence
- Cell Line
- DNA Replication
- DNA, Viral
- Genetic Techniques
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Plasmids
- Proviruses
- Repetitive Sequences, Nucleic Acid
- Repressor Proteins
- Retroviridae
- Transfection