Escherichia coli endonuclease VIII: cloning, sequencing, and overexpression of the nei structural gene and characterization of nei and nei nth mutants.

Abstract excerpt

Escherichia coli possesses two DNA glycosylase/apurinic lyase activities with overlapping substrate specificities, endonuclease III and endonuclease VIII, that recognize and remove oxidized pyrimidines from DNA. Endonuclease III is encoded by the nth gene. Endonuclease VIII has now been purified...

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• Deoxyribonuclease (Pyrimidine Dimer)
• Endodeoxyribonucleases
• Escherichia coli
• Escherichia coli Proteins
• Gene Expression Regulation, Bacterial
• Genes, Bacterial
• Molecular Sequence Data
• Mutation
• Sequence Alignment