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Article

Improving the tolerance of a protein a analogue to repeated alkaline exposures using a bypass mutagenesis approach.

Proteins - 1 May 2004

Linhult Martin, Gülich Susanne, Gräslund Torbjörn, Simon Annelie, Karlsson Martin, Sjöberg Anna, Nord Karin, Hober Sophia

Abstract excerpt

Staphylococcal protein A (SPA) is a cell surface protein expressed by Staphylococcus aureus. It consists of five repetitive domains. The five SPA-domains show individual interaction to the Fc-fragment as well as certain Fab-fragments of immunoglobulin G (IgG) from most mammalian species. Due to the high affinity and selectivity of SPA, it has a widespread use as an affinity ligand for capture and purification of...

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Identifiers

PMID: 15048831
DOI: 10.1002/prot.10616

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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