Article

Challenges in Expression and Purification of Functional Fab Fragments in E. coli: Current Strategies and Perspectives

2022-04-09

Abstract excerpt

Microbial host systems remain the most efficient and cost-effective chassis for biotherapeutics production. Escherichia coli is often the preferred host due to ease of cloning, scale-up, high product yields, and most importantly, cost-effective cultivation. E. coli often experience difficulties in producing biologically active therapeutics such as Fab fragments, which require protein folding and subsequent three-d...

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Literature Corpus work: d79ccac8-4bc5-5ae7-818a-939a059b0f4d
DOI: 10.3390/fermentation8040175

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