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Article

Protein engineering of an IgG-binding domain allows milder elution conditions during affinity chromatography.

Journal of biotechnology - 21 Jan 2000

Gülich S, Uhlén M, Hober S

Abstract excerpt

One of the problems in the recovery of antibodies by affinity chromatography is the low pH, which is normally essential to elute the bound material from the column. Here, we have addressed this problem by constructing destabilized mutants of a domain analogue (domain Z) from an IgG-binding bacter...

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Identifiers

PMID: 10656338
DOI: 10.1016/s0168-1656(99)00197-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-10656338-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

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