Article
Fine affinity discrimination by normalized fluorescence activated cell sorting in staphylococcal surface display.
FEMS microbiology letters - 15 Jul 2005
Löfblom John, Wernérus Henrik, Ståhl Stefan
Abstract excerpt
We have investigated a staphylococcal surface display system for its potential future use as a protein library display system in combinatorial biochemistry. Efficient affinity-based selections require a system capable of fine affinity discrimination of closely related binders to minimize the loss of potentially improved variants. In this study, a significant breakthrough was achieved to avoid biases due to...
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