Structure/function analysis of interleukin-2-toxin (DAB486-IL-2). Fragment B sequences required for the delivery of fragment A to the cytosol of target cells.

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Article

Structure/function analysis of interleukin-2-toxin (DAB486-IL-2). Fragment B sequences required for the delivery of fragment A to the cytosol of target cells.

The Journal of biological chemistry - 15 Jul 1990

Williams D P, Snider C E, Strom T B, Murphy J R

Abstract excerpt

We have used cassette and deletion mutagenesis to analyze the structural features of fragment B-related sequences in the fusion toxin DAB486-IL-2 (where IL-2 represents interleukin-2) that are necessary for the efficient delivery of fragment A to the cytosol of target cells. We demonstrate that whereas an intact disulfide bond between Cys461 and Cys471 may be required for the cytotoxic action of native diphtheria...

Topics

Animals
Binding, Competitive
Biological Transport
Cell Line
Cell Survival
Cloning, Molecular
Cystine
Cytosol
Diphtheria Toxin
Escherichia coli
Immunotoxins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Interleukin-2

Kinetics

Mutation

Plasmids

Receptors, Interleukin-2

Recombinant Fusion Proteins

Restriction Mapping

Source

Open in PubMed

Identifiers

PMID: 2195027

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2195027-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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