Article
Regulation of yeast LEU2. Total deletion of regulatory gene LEU3 unmasks GCN4-dependent basal level expression of LEU2.
The Journal of biological chemistry - 15 Jul 1990
Brisco P R, Kohlhaw G B
Abstract excerpt
We have constructed a total deletion of the regulatory gene LEU3. Comparing the deletion mutant with a leu3 spontaneous mutant, we find that both types of mutants have lost the ability to regulate a LEU2'-lacZ translational fusion by the LEU3-alpha-isopropylmalate-dependent mechanism, which we confirm to be the major regulatory mechanism for LEU2. Surprisingly, cells containing the total leu3 deletion are more...
Topics
- 2-Isopropylmalate Synthase
- Base Sequence
- Chromosome Deletion
- Escherichia coli
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genes, Regulator
- Leucine
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes