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Article

Dissecting electrostatic interactions in Bacillus circulans xylanase through NMR-monitored pH titrations.

Journal of biomolecular NMR - 1 Sept 2011

McIntosh Lawrence P, Naito Daigo, Baturin Simon J, Okon Mark, Joshi Manish D, Nielsen Jens E

Abstract excerpt

NMR-monitored pH titration curves of proteins provide a rich source of structural and electrostatic information. Although relatively straightforward to measure, interpreting pH-dependent chemical shift changes to obtain site-specific acid dissociation constants (pK (A) values) is challenging. In order to analyze the biphasic titrations exhibited by the side chain (13)C(γ) nuclei of the...

Topics

Bacillus
Bacterial Proteins
Binding Sites
Biocatalysis
Endo-1,4-beta Xylanases
Glutamic Acid
Hydrogen-Ion Concentration
Kinetics
Mutation
Nuclear Magnetic Resonance, Biomolecular

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Static Electricity

Source

Identifiers

PMID: 21947911
DOI: 10.1007/s10858-011-9537-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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