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Article

A method to determine residue-specific unfolded-state pKa values from analysis of stability changes in single mutant cycles.

Journal of the American Chemical Society - 2 Jun 2010

Shen Jana K

Abstract excerpt

It is now widely recognized that the unfolded state of a protein in equilibrium with the native state under folding conditions may contain significant residual structures. However, due to technical difficulties residue-specific interactions in the unfolded state remain elusive. Here we introduce a method derived from the Wyman-Tanford theory to determine residue-specific pK(a)'s in the unfolded state. This method...

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Identifiers

PMID: 20446661
DOI: 10.1021/ja101761m

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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