Article
Protein overexpression following lentiviral infection of primary mature neutrophils is due to pseudotransduction.
Journal of immunological methods - 28 Oct 2011
Geering Barbara, Schmidt-Mende Jan, Federzoni Elena, Stoeckle Christina, Simon Hans-Uwe
Abstract excerpt
Neutrophils are terminally differentiated cells with a short life-span due to constitutive apoptosis. Because of these characteristics, genetic manipulation of neutrophils has been difficult, although it is highly desired given the importance of neutrophils in the immune system. Here we demonstrate that transduction of primary human mature neutrophils with enhanced green fluorescent protein (eGFP)-encoding...
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