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Article

In vitro and in vivo activities of T4 endonuclease V mutants altered in the C-terminal aromatic region.

Biochemistry - 24 Apr 1990

Ishida M, Kanamori Y, Hori N, Inaoka T, Ohtsuka E

Abstract excerpt

Genes encoding mutants of the thymine photodimer repair enzyme from bacteriophage T4 (T4 endonuclease V) having an amino acid substitution (T127M, W128A, W128S, Y129A, K130L, Y131A, Y132A) were constructed by use of a previously obtained synthetic gene and expressed in Escherichia coli under the control of the E. coli tryptophan promoter. An in vitro assay of partially fractionated mutant proteins for glycosylase...

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PMID: 2191719
DOI: 10.1021/bi00468a002

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