Article
[Effect of gamma-glutamyl kinase gene knock-out on metabolism in L-arginine-producing strain Corynebacterium crenatum 8-193].
Wei sheng wu xue bao = Acta microbiologica Sinica - 4 Nov 2011
Li Xiaoman, Zhao Zhi, Zhang Yingzi, Wang Yu, Ding Jiuyuan
Abstract excerpt
OBJECTIVE: In order to optimize precursor supply for L-arginine biosynthesis, we constructed a Corynebacterium crenatum 8-193 mutant with gamma-glutamyl kinase gene (proB) in-frame deletion. The effects of proB knock-out on physiological characteristics of the mutant were investigated. METHODS: The upstream and downstream fragments of proB were cloned from C. crenatum 8-193 chromosome and ligated to integration...
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