Article
An improved flow cytometric assay for somatic mutations at the glycophorin A locus in humans.
Cytometry - 1 Jan 1990
Langlois R G, Nisbet B A, Bigbee W L, Ridinger D N, Jensen R H
Abstract excerpt
An improved method has been developed for the glycophorin A assay for somatic cell mutations in humans. The new assay, named the "BR6" assay, can be performed on a commercially available, single-beam flow cytometer, in contrast to the previously described 1W1 assay that required a dual-beam flow sorter. A modified cell labeling method developed for the BR6 assay results in improved separation of normal and mutant...
Topics
- Adult
- Antibodies, Monoclonal
- Cesium Radioisotopes
- Cytogenetics
- Female
- Flow Cytometry
- Fluorescent Antibody Technique
- Gamma Rays
- Genetic Variation
- Glycophorins
- Humans