Article
High gradient magnetic cell sorting and internal standardisation substantially improve the assay for somatic mutations at the glycophorin A (GPA) locus.
Mutation research - 9 Apr 2003
Hempel Klaus, Deubel Werner, Lorenz Reinhard, Reiners Christoph
Abstract excerpt
In the MACS-BR6 version of the GPA assay [Int. J. Radiat. Res. 70 (1996) 131] variant red blood cells (RBC) are isolated from 5 x 10(8) normal RBC by magnetic cell separation (MACS) before detection and quantification by immunolabelling and flow cytometry as in the classical BR6 assay. In the present work it is described how the MACS-BR6 assay is improved by internal standardisation with FITC-labelled RBC. This...
Topics
- Adult
- Antigens, Tumor-Associated, Carbohydrate
- Cell Separation
- Erythrocytes
- Flow Cytometry
- Fluorescein-5-isothiocyanate
- Fluorescent Dyes
- Genetic Variation
- Glycophorins
- Humans