Article
Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.
Molecular and cellular biology - 1 Sept 2013
Egriboz Onur, Goswami Sudip, Tao Xiaorong, Dotts Kathleen, Schaeffer Christie, Pilauri Vepkhia, Hopper James E
Abstract excerpt
The DNA-binding transcriptional activator Gal4 and its regulators Gal80 and Gal3 constitute a galactose-responsive switch for the GAL genes of Saccharomyces cerevisiae. Gal4 binds to GAL gene UASGAL (upstream activation sequence in GAL gene promoter) sites as a dimer via its N-terminal domain and activates transcription via a C-terminal transcription activation domain (AD). In the absence of galactose, a Gal80...
Topics
- Binding, Competitive
- DNA-Binding Proteins
- Galactose
- Genes, Fungal
- Mutation
- Protein Binding
- Protein Interaction Domains and Motifs
- Protein Multimerization
- Protein Structure, Quaternary
- Recombinant Fusion Proteins
- Repressor Proteins
- Saccharomyces cerevisiae