Article
Gal80 dimerization and the yeast GAL gene switch.
Genetics - 1 Apr 2005
Pilauri Vepkhia, Bewley Maria, Diep Cuong, Hopper James
Abstract excerpt
The Saccharomyces cerevisiae Gal80 protein has two binding partners: Gal4 and Gal3. In the absence of galactose, Gal80 binds to and inhibits the transcriptional activation domain (AD) of the GAL gene activator, Gal4, preventing GAL gene expression. Galactose triggers an association between Gal3 and Gal80, relieving Gal80 inhibition of Gal4. We selected for GAL80 mutants with impaired capacity of Gal80 to bind to...
Topics
- DNA Primers
- DNA-Binding Proteins
- Dimerization
- Fungal Proteins
- Galactose
- Gene Expression Regulation, Fungal
- Models, Biological
- Models, Genetic
- Models, Molecular
- Mutagenesis
- Mutation
- Mutation, Missense