Article
Improved expression of human interleukin-2 in high-cell-density fermentor cultures of Escherichia coli K-12 by a phosphotransacetylase mutant.
Applied and environmental microbiology - 1 May 1990
Bauer K A, Ben-Bassat A, Dawson M, de la Puente V T, Neway J O
Abstract excerpt
A fluoroacetate-resistant mutant of Escherichia coli K-12 (MM-294) accumulated less acetate in the medium during growth to high cell density in fermentor cultures and was shown to be defective in its phosphotransacetylase activity. The mutant had an improved ability to continue growing during induction of interleukin-2 (IL-2) synthesis, and in fermentor cultures it gave a higher level of specific IL-2...
Topics
- Acetates
- Acetyltransferases
- Escherichia coli
- Ethanol
- Fermentation
- Interleukin-2
- Mutation
- Phosphate Acetyltransferase
- Pyruvates
- Pyruvic Acid