Article
Effects of propeptide deletion on human renin secretion from mouse pituitary AtT-20 cells.
FEBS letters - 7 May 1990
Nagahama M, Nakayama K, Murakami K
Abstract excerpt
To study the role of the N-terminal propeptide in the secretory process of renin, mouse pituitary AtT-20 cells were transfected with expression plasmids of human preprorenin and a mutant deleted of its propeptide. The transfectant of the native construct secreted inactive prorenin and active renin, and renin secretion was stimulated by a secretagogue, 8-Br-cAMP. On the contrary, the transfectant of the deleted...
Topics
- Animals
- Cell Line
- Chromosome Deletion
- Enzyme Precursors
- Humans
- Kinetics
- Mice
- Mutation
- Oligonucleotide Probes
- Pituitary Neoplasms
- Plasmids
- Renin