Article
The third transmembrane segment of orai1 protein modulates Ca2+ release-activated Ca2+ (CRAC) channel gating and permeation properties.
The Journal of biological chemistry - 7 Oct 2011
Srikanth Sonal, Yee Ma-Khin Win, Gwack Yousang, Ribalet Bernard
Abstract excerpt
Orai1, the pore subunit of Ca(2+) release-activated Ca(2+) channels, has four transmembrane segments (TMs). The first segment, TMI, lines the pore and plays an important role in channel activation and ion permeation. TMIII, on the other hand, does not line the pore but still regulates channel gating and permeation properties. To understand the role of TMIII, we have mutated and characterized several residues in...
Topics
- Amino Acid Sequence
- Animals
- Calcium
- Calcium Channels
- Calcium Signaling
- Cell Membrane
- Humans
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutation
- NFATC Transcription Factors
- ORAI1 Protein
- Patch-Clamp Techniques