Article
Fluorescence dequenching makes haem-free soluble guanylate cyclase detectable in living cells.
PloS one - 1 Jan 2011
Hoffmann Linda S, Schmidt Peter M, Keim Yvonne, Hoffmann Carsten, Schmidt Harald H H W, Stasch Johannes-Peter
Abstract excerpt
In cardiovascular disease, the protective NO/sGC/cGMP signalling-pathway is impaired due to a decreased pool of NO-sensitive haem-containing sGC accompanied by a reciprocal increase in NO-insensitive haem-free sGC. However, no direct method to detect cellular haem-free sGC other than its activation by the new therapeutic class of haem mimetics, such as BAY 58-2667, is available. Here we show that fluorescence...
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