Article
A comprehensive HBV array for the detection of HBV mutants and genotype.
Clinical biochemistry - 1 Oct 2011
Chan Kaimin, Yam Irene, Yuen John, Yuen Man-Fung, Lai Ching-Lung, Alexander Graeme J, Chan Tai-Kwong, Chan Vivian
Abstract excerpt
OBJECTIVE: To develop a comprehensive hepatitis B virus (HBV) array providing simultaneous analysis of 8 genotypes, 47 mutations of reverse-transcriptase polymerase gene and 18 mutations of S gene. METHOD: Oligonucleotides corresponding to various HBV-normal and -mutant sequences were spotted onto pre-treated glass slides. Single-stranded templates of the HBV gene fragment were prepared from serum-DNA of...
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