Article
Identification of kinase substrates by bimolecular complementation assays.
The Plant journal : for cell and molecular biology - 1 Apr 2012
Pusch Stefan, Harashima Hirofumi, Schnittger Arp
Abstract excerpt
As a consequence of the transient nature of kinase-substrate interactions, the detection of kinase targets, although central for understanding many biological processes, has remained challenging. Here we present a straightforward procedure that relies on the comparison of wild type with activation-loop mutants in the kinase of interest by bimolecular complementation assays. As a proof of functionality, we present...
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