Article
The G1613A mutation in the HBV genome affects HBeAg expression and viral replication through altered core promoter activity.
PloS one - 1 Jan 2011
Li Man-Shan, Lau Terrence Chi-Kong, Chan Sophie Ka-Ping, Wong Chi-Hang, Ng Patrick Kwok-Shing, Sung Joseph Jao-Yiu, Chan Henry Lik-Yuen, Tsui Stephen Kwok-Wing
Abstract excerpt
Infection of hepatitis B virus (HBV) causes acute and chronic hepatitis and is closely associated with the development of cirrhosis and hepatocellular carcinoma (HCC). Previously, we demonstrated that the G1613A mutation in the HBV negative regulatory element (NRE) is a hotspot mutation in HCC patients. In this study, we further investigated the functional consequences of this mutation in the context of the full...
Topics
- Blotting, Northern
- Blotting, Southern
- Blotting, Western
- Carcinoma, Hepatocellular
- DNA, Viral
- DNA-Binding Proteins
- Electrophoretic Mobility Shift Assay
- Enzyme-Linked Immunosorbent Assay
- Female