Article
Reverse transcriptases and genomic variability: the accuracy of DNA replication is enzyme specific and sequence dependent.
The EMBO journal - 1 May 1990
Ricchetti M, Buc H
Abstract excerpt
Kinetics of incorporation of correct and incorrect deoxynucleotides by three reverse transcriptases have been followed, by gel assay, on a series of DNA templates, including part of the HIV-1 gag DNA minus strand. Insertion kinetics for the properly matched nucleotide at a given place on the template vary strongly from one enzyme to the next. No significant correlation is found between the site-specific Michaelis...
Topics
- Avian Myeloblastosis Virus
- Base Sequence
- DNA Replication
- DNA, Viral
- Deoxyribonucleotides
- Gene Products, gag
- Genetic Variation
- HIV-1
- Kinetics
- Molecular Sequence Data
- Moloney murine leukemia virus