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Article

Evidence for preferential genotyping of a minority human immunodeficiency virus population due to primer-template mismatching during PCR-based amplification.

Journal of clinical microbiology - 1 Jan 2005

Martinez-Picado Javier, Morales-Lopetegi Kristina, Villena Cristina, Gutiérrez Carolina, Izquierdo Nuria, Marfil Silvia, Clotet Bonaventura, Ruiz Lidia

Abstract excerpt

Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of the original viral population in the sample, magnifying a distinct minority HIV-1 population. This observation might help to explain some unexpected HIV-1 genotypes.

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Drug Resistance, Viral

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Share to Nucleic Acid Amplification Techniques

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Source

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Identifiers

PMID: 15635008
DOI: 10.1128/JCM.43.1.436-438.2005
PMCID: PMC540107

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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