Article
Packaging signals in the 5'-ends of influenza virus PA, PB1, and PB2 genes as potential targets to develop nucleic-acid based antiviral molecules.
Antiviral research - 1 Oct 2011
Giannecchini Simone, Wise Helen M, Digard Paul, Clausi Valeria, Del Poggetto Edoardo, Vesco Liberio, Puzelli Simona, Donatelli Isabella, Azzi Alberta
Abstract excerpt
In a previous study a 15-mer phosphorothioate oligonucleotide (S-ON) derived from the packaging signal in the 5' end of segment 1 (PB2) of influenza A virus (designated 5-15b) proved markedly inhibitory to virus replication. Here we investigated whether analogous inhibitory S-ONs targeting the 5' end of segments 2 (PB1) and 3 (PA) could be identified and whether viral resistance to S-ONs can be developed. Similar...
Topics
- Animals
- Antiviral Agents
- Cell Line
- Drug Resistance, Multiple, Viral
- Humans
- Influenza A Virus, H1N1 Subtype
- Influenza A virus
- Mutation
- Phosphorothioate Oligonucleotides
- RNA, Viral
- RNA-Dependent RNA Polymerase
- Viral Proteins