Article
A filamentous-like mutant of Listeria monocytogenes with reduced expression of a 60-kilodalton extracellular protein invades and grows in 3T6 and Caco-2 cells.
Canadian journal of microbiology - 1 Aug 1992
Gutekunst K A, Pine L, White E, Kathariou S, Carlone G M
Abstract excerpt
We describe a spontaneous rough mutant of Listeria monocytogenes that produces reduced amounts of a 60-kilodalton major extracellular polypeptide (p60) as shown by sodium dodecyl sulfate--polyacrylamide gel electrophoresis and Western blot analysis. The cells of this mutant are filamentous, do not give rise to smooth wild-type colonies, and produce listeriolysin O in amounts equal to that of the wild-type cells,...
Topics
- Animals
- Bacterial Proteins
- Cell Line
- Humans
- Listeria monocytogenes
- Mice
- Microbiological Techniques
- Microscopy, Electron
- Molecular Weight
- Mutation
- Phenotype