Article
A role for noncanonical microRNAs in the mammalian brain revealed by phenotypic differences in Dgcr8 versus Dicer1 knockouts and small RNA sequencing.
RNA (New York, N.Y.) - 1 Aug 2011
Babiarz Joshua E, Hsu Ruby, Melton Collin, Thomas Molly, Ullian Erik M, Blelloch Robert
Abstract excerpt
Noncanonical microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs) are distinct subclasses of small RNAs that bypass the DGCR8/DROSHA Microprocessor but still require DICER1 for their biogenesis. What role, if any, they have in mammals remains unknown. To identify potential functional properties for these subclasses, we compared the phenotypes resulting from conditional deletion of Dgcr8 versus...
Topics
- Animals
- Brain
- DEAD-box RNA Helicases
- Mice
- Mice, Knockout
- MicroRNAs
- Mitosis
- Phenotype
- Proteins
- RNA-Binding Proteins
- Ribonuclease III
- Sequence Analysis, RNA