Article
Recombinant phenotyping of cytomegalovirus UL54 mutations that emerged during cell passages in the presence of either ganciclovir or foscarnet.
Antimicrobial agents and chemotherapy - 1 Sept 2011
Gilbert Christian, Azzi Arezki, Goyette Nathalie, Lin Sheng-Xiang, Boivin Guy
Abstract excerpt
Selection of human cytomegalovirus variants in the presence of ganciclovir or foscarnet led to 18 DNA polymerase mutations, 14 of which had not been previously studied. Using bacterial artificial chromosome technology, each of these mutations was individually transferred into the genome of a reference strain. Following reconstitution of infectious viral stocks, each mutant was assessed for its drug susceptibility...
Topics
- Antiviral Agents
- Cells, Cultured
- Cytomegalovirus
- DNA-Directed DNA Polymerase
- Foscarnet
- Ganciclovir
- Genotype
- Humans
- Mutation
- Phenotype
- Recombination, Genetic
- Viral Proteins