Article
Mutational and structural studies of the PixD BLUF output signal that affects light-regulated interactions with PixE.
Biochemistry - 26 Jul 2011
Yuan Hua, Dragnea Vladimira, Wu Qiong, Gardner Kevin H, Bauer Carl E
Abstract excerpt
PixD (Slr1694) is a BLUF (blue-light-using FAD) photoreceptor used by the cyanobacterium Synechocystis sp. PCC6803 to control phototaxis toward blue light. In this study, we probe the involvement of a conserved Tyr8-Gln50-Met93 triad in promoting an output signal upon blue light excitation of the bound flavin. Analysis of acrylamide quenching of Trp91 fluorescence shows that the side chain of this residue remains...
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