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Article

A Q63E Rhodobacter sphaeroides AppA BLUF domain mutant is locked in a pseudo-light-excited signaling state.

Biochemistry - 21 Dec 2010

Dragnea Vladimira, Arunkumar Alphonse I, Lee Chul Won, Giedroc David P, Bauer Carl E

Abstract excerpt

The AppA BLUF photoreceptor from Rhodobacter sphaeroides contains a conserved key residue, Gln63, that is thought to undergo a shift in hydrogen-bonding interactions when a bound flavin is light excited. In this study we have characterized two substitution mutants of Gln63 (Q63E, Q63L) in the context of two constructs of the BLUF domain that have differing lengths, AppA1-126 and AppA17-133. Q63L mutations in both...

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Identifiers

PMID: 21082791
DOI: 10.1021/bi1002162
PMCID: PMC3127458

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Source receipt: pubmed, retrieved 7 Sept 2026.

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