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Article

[Codon optimization of recombinant staphylococcal enterotoxin O enhances the expression level in Escherichia coli].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences - 1 May 2011

Huang Peng, Sun Hong-ying, Chen Shu-qing

Abstract excerpt

OBJECTIVE: To enhance the expression level of staphylococcal enterotoxin O (SEO) by optimization of rare codons. METHODS: The gene of mature SEO (His-tag included) was cloned to pET28a, and 15 rare codons on the gene were optimized by PCR technology. These recombinant plasmids then were transformed into E.coli BL21(DE3), respectively. After IPTG induced, the expression levels of those mutants were analyzed by...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 21671491
DOI: 10.3785/j.issn.1008-9292.2011.03.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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