Article
[Clone, construct, expression and verification of lactoferricin B gene and several sequence mutations in yeast].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition - 1 Jul 2007
Feng Yong-qian, Zha Xiao-jun, Zhai Chao-yang
Abstract excerpt
OBJECTIVE: To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB expressing in yeast of S. cerevisiae, of which the expressed protein antibacterial activity was verified in preliminary. METHODS: By self-template PCR method, the gene of Lactoferricin B and its several sequence mutations were amplified with the parts of the pre-synthesized single chains. And then Lactoferricin B gene and its...
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