Article
[Improving production and bioactivity of recombinant human disintegrin domain of ADAM15 (rhADAM15) in Escherichia coli].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Aug 2008
Wu Jing, Lei Jianyong, Zhang Lianfen, Hua Hui, Jin Jian
Abstract excerpt
OBJECTIVE: To enhance the production and bioactivity of recombinant human disintegrin domain of ADAM15 (A Disintegrin and Metalloproteinase 15) (rhADAM15) in Escherichia coli. METHODS: The expression host was chose and the recombinant expression plasmid pGEX-ADAM15 was constructed, based on analysis of the cDNA sequence of rhADAM15. RESULTS: (1) 298 mg/L GST (Glutathione-S-transferase)-ADAM15 and 42 mg/L rhADAM15...
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