Article
Precise excision and instability of the transposon Tn5 in Pseudomonas aeruginosa.
Journal of general microbiology - 1 May 1990
Goldberg J B, Won J, Ohman D E
Abstract excerpt
Excision of the transposon Tn5 from sites of insertion in plasmid DNA was shown to occur at high frequency in Pseudomonas aeruginosa. Plasmids with Tn5 insertions conjugated poorly into P. aeruginosa and adversely affected growth compared to the respective parental plasmids. The kanamycin-resistance phenotype of Tn5 was expressed poorly in P. aeruginosa and kanamycin-sensitive strains were common during the...
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