Precise and nearly-precise excision of the symmetrical inverted repeats of Tn5; common features of recA-independent deletion events in Escherichia coli.

doi:10.1016/0378-1119(82)90198-6

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Article

Precise and nearly-precise excision of the symmetrical inverted repeats of Tn5; common features of recA-independent deletion events in Escherichia coli.

Gene - 1 Jan 2000

Collins J, Volckaert G, Nevers P

Abstract excerpt

The transposon Tn5 contains a unique central region bordered by 1.5-kb inverted repeats. The in vitro deletion of the centre of Tn5, with a restriction endonuclease (XhoI) which cuts within the inverted repeats leads to the production of a palindrome on subsequent ligation. This palindromic regio...

Topics

Bacterial Proteins
Base Sequence
Chromosome Deletion
DNA Restriction Enzymes
DNA Transposable Elements
Escherichia coli
Genotype
Plasmids
Rec A Recombinases
Repetitive Sequences, Nucleic Acid
Species Specificity

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