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Article

Isolation of high frequency of recombination donors from Tn5 chromosomal mutants of Pseudomonas putida PPN and recalibration of the genetic map.

Genetics - 1 Nov 1990

Strom A D, Hirst R, Petering J, Morgan A

Abstract excerpt

A Tn5 loaded derivative of the IncP-10 plasmid R91-5 (pMO75) was used as a suicide vector to generate random chromosomal insertion mutations in Pseudomonas putida PPN. Reintroduction of pMO75 into such mutants resulted in integration of the plasmid at the site of Tn5 insertion, giving rise to two classes of high frequency of donors recombination (Hfr) donors, transferring chromosome at high frequency (greater...

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Identifiers

PMID: 2174392
DOI: 10.1093/genetics/126.3.497
PMCID: PMC1204207

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