Isolation of high frequency of recombination donors from Tn5 chromosomal mutants of Pseudomonas putida PPN and recalibration of the genetic map.

doi:10.1093/genetics/126.3.497

Back to search

Article

Isolation of high frequency of recombination donors from Tn5 chromosomal mutants of Pseudomonas putida PPN and recalibration of the genetic map.

Genetics - 1 Nov 1990

Strom A D, Hirst R, Petering J, Morgan A

Abstract excerpt

A Tn5 loaded derivative of the IncP-10 plasmid R91-5 (pMO75) was used as a suicide vector to generate random chromosomal insertion mutations in Pseudomonas putida PPN. Reintroduction of pMO75 into such mutants resulted in integration of the plasmid at the site of Tn5 insertion, giving rise to two...

Topics

Blotting, Southern
Chromosome Mapping
DNA Transposable Elements
Kinetics
Mutation
Oligopeptides
Pigments, Biological
Pseudomonas
Recombination, Genetic

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.