Article
Insertional mutagenesis of Listeria monocytogenes 568 reveals genes that contribute to enhanced thermotolerance.
International journal of food microbiology - 30 Nov 2009
Ells Timothy C, Speers R Alex, Hansen Lisbeth Truelstrup
Abstract excerpt
The objectives of this study were to identify molecular mechanisms of thermotolerance using transposon mutants of Listeria monocytogenes 568, serotype 1/2a, and to compare their thermal death kinetics at 52, 56 and 60 degrees C. Sixteen Tn917 transposon mutants with enhanced heat resistance were acquired from a library of 4300 mutants following a multi-step screening process. Genetic regions with Tn917 insertions...
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