Article
PCR-free digital minisatellite tandem repeat genotyping.
Electrophoresis - 1 Jun 2011
Chen Yuchao, Seo Tae Seok
Abstract excerpt
We demonstrated a proof-of-concept for novel minisatellite tandem repeat typing, called PCR-free digital VNTR (variable number tandem repeat) typing, which is composed of three steps: a ligation reaction instead of PCR thermal cycling, magnetic bead-based solid-phase capture for purification, and an elongated sample stacking microcapillary electrophoresis (μCE) for sensitive digital coding of repeat number. We...
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